How Recombinant Proteins are Expressed in Escherichia coli

The successful expression of recombinant proteins in Escherichia coli (E. coli) is one of the central advancements in biosciences. E. coli has become a standard host in biotechnology because of its easy genetic tractability, rapid growth rate, and cost-effectiveness. Used for the production of a diverse array of proteins, enzymes, hormones, and pharmaceutical-grade antibodies, this capability of the microorganism has fueled academic study. We will understand how rProteins are expressed in E.coli and what makes this tech an essential part of modern research. Mechanism of Recombinant Protein Expression Before we understand the inside-out mechanism of recombinant protein expression, we need to understand what recombinant proteins are. At the core, recombinant proteins are artificially produced proteins that are produced by introducing foreign genes into an organism. The gene encoding the target protein is inserted into E.coli, and the cellular mechanism of the...